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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
11/08/2016 |
Actualizado : |
12/03/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
ROCHA, C.M.L.; VELLICCE, G.R.; GARCÍA, M.G.; PARDO, E.M.; RACEDO, J.; PERERA, M.F.; DE LUCÍA, A.; BONNECARRERE, M.; GERMAN, S.; MARCELINO, F.; LEDESMA, F.; REZNIKOV, S.; PLOPER, D.L.; WELIN, B.; CASTAGNARO, A.P. |
Afiliación : |
CARLA MARÍA LOURDES ROCHA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; GABRIEL RICARDO VELLICCE, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; MARÍA GABRIELA GARCÍA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ESTEBAN MARIANO PARDO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; JOSEFINA RACEDO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; MARÍA FRANCISCA PERERA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ADRIÁN DE LUCÍA, INTA (Instituto Nacional de Tecnología Agropecuaria) - Cerro Azul; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANCISMAR MARCELINO, EMBRAPA Soja; FRANCISCO LEDESMA, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; SEBASTIÁN REZNIKOV, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; LEONARDO DANIEL PLOPER, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; BJORN WELIN, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino; ATILIO PEDRO CASTAGNARO, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Tecnología Agroindustrial del Noroeste Argentino. |
Título : |
Use of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2015, v. 18, no. 6, p. 439-444. OPEN ACCESS. |
DOI : |
10.1016/j.ejbt.2015.06.007 |
Idioma : |
Inglés |
Notas : |
Article history: Received 24 April 2015. Accepted 23 September 2015. Available online 28 October 2015. |
Contenido : |
ABSTRACT.
Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919
markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies.
© 2015 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserve MenosABSTRACT.
Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919
markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an impor... Presentar Todo |
Palabras claves : |
ASIAN SOYBEAN RUST; GENETIC VARIATION; GLYCINE MAX; MOLECULAR MARKERS. |
Thesagro : |
MARCADORES MOLECULARES; SOJA; VARIACION GENETICA. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5850/1/Rocha-C.M.-2015.-Electr.Jr.Biotech.-v18-p.439-444.pdf
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Marc : |
LEADER 02957naa a2200397 a 4500 001 1055241 005 2019-03-12 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1016/j.ejbt.2015.06.007$2DOI 100 1 $aROCHA, C.M.L. 245 $aUse of AFLP markers to estimate molecular diversity of Phakopsora pachyrhizi.$h[electronic resource] 260 $c2015 500 $aArticle history: Received 24 April 2015. Accepted 23 September 2015. Available online 28 October 2015. 520 $aABSTRACT. Background: Asian soybean rust (SBR) caused by Phakopsora pachyrhizi Syd. & Syd., is one of the main diseases affecting soybean and has been reported as one of the most economically important fungal pathogens worldwide. Knowledge of the genetic diversity of this fungus should be considered when developing resistance breeding strategies. We aimed to analyze the genetic diversity of P. pachyrhizi combining simple sampling with a powerful and reproducible molecular technique. Results: We employed Amplified Fragment Length Polymorphism (AFLP) technique for the amplification of P. pachyrhizi DNA extracted from naturally SBR-infected plants from 23 production fields. From a total of 1919 markers obtained, 77% were polymorphic. The high percentage of polymorphism and the Nei's genetic diversity coefficient (0.22) indicated high pathogen diversity. Analysis of molecular variance showed higher genetic variation within countries than among them. Temporal analysis showed a higher genetic variation within a year than between years. Cluster, phylogenetic and principal co-ordinate analysis showed that samples group by year of collection and then by country sampled. Conclusions: The study proposed combining a simple collection of urediniospore with a subsequent analysis by AFLP was useful to examine the molecular polymorphism of samples of P. pachyrhizi collected and might have a significant contribution to the knowledge of its genetic diversity. Also, AFLP analysis is an important and potent molecular tool for the study of genetic diversity and could be useful to carry out wider genetic diversity studies. © 2015 Pontificia Universidad Católica de Valparaíso. Production and hosting by Elsevier B.V. All rights reserve 650 $aMARCADORES MOLECULARES 650 $aSOJA 650 $aVARIACION GENETICA 653 $aASIAN SOYBEAN RUST 653 $aGENETIC VARIATION 653 $aGLYCINE MAX 653 $aMOLECULAR MARKERS 700 1 $aVELLICCE, G.R. 700 1 $aGARCÍA, M.G. 700 1 $aPARDO, E.M. 700 1 $aRACEDO, J. 700 1 $aPERERA, M.F. 700 1 $aDE LUCÍA, A. 700 1 $aBONNECARRERE, M. 700 1 $aGERMAN, S. 700 1 $aMARCELINO, F. 700 1 $aLEDESMA, F. 700 1 $aREZNIKOV, S. 700 1 $aPLOPER, D.L. 700 1 $aWELIN, B. 700 1 $aCASTAGNARO, A.P. 773 $tElectronic Journal of Biotechnology, 2015$gv. 18, no. 6, p. 439-444. OPEN ACCESS.
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
30/01/2024 |
Actualizado : |
30/01/2024 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
ALDABE, J.; MORÁN-LÓPEZ, T.; SOCA, P.; BLUMETTO, O.; MORALES, J.M. |
Afiliación : |
JOAQUÍN ALDABE, Departamento de Sistemas Agrarios y Paisajes Culturales, Centro Universitario Regional del Este, Universidad de la República, Rocha, Uruguay; Southern Cone Grassland Alliance, Aves Uruguay-BirdLife International, Montevideo, Uruguay; TERESA MORÁN-LÓPEZ, Dpto. Biología de Organismos y Sistemas, Univ. Oviedo & Intst. Mixto de Inv. en Biodiversidad (Univ. de Oviedo-CSIC-Principado de Asturias), Oviedo & Mieres, Spain: Grupo Ecología Cuantitativa, INIBIOMA-CONICET, Univ. Nac. Comahue, Bs.As., Argentina; PABLO SOCA, Ecología del Pastoreo Group, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; OSCAR RICARDO BLUMETTO VELAZCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JUAN MANUEL MORALES, Grupo de Ecología Cuantitativa, INIBIOMA-CONICET, Universidad Nacional del Comahue, Bariloche, Argentina; School of Biodiversity, One Health and Veterinary Medicine, University of Glasgow, Glasgow, United Kingdom. |
Título : |
Bird species responses to rangeland management in relation to their traits: Rio de la Plata Grasslands as a case study. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Ecological Applications, 2023, ee2933. https://doi.org/10.1002/eap.2933 |
ISSN : |
1051-0761 |
DOI : |
10.1002/eap.2933 |
Idioma : |
Inglés |
Notas : |
Article history: Manuscript received: 25 April 2023; Manuscript revised: 31 August 2023; Manuscript accepted: 04 October 2023; Accepted manuscript online: 20 November 2023; Version of Record online: 20 December 2023. -- Correspondence: Aldabe, J.; Departamento de Sistemas Agrarios y Paisajes Culturales, Centro Universitario Regional del Este, Universidad de la República, Rocha, Uruguay; email:joaquin.aldabe@gmail.com -- Funding: This paper was developed in the context of J. Aldabe PhD at Ciencias Agrarias Postgraduate Program, Agronomy Faculty, Universidad de la República. TML was supported by a Juan de la Cierva Incorporación Fellowship (IJC2020-043765-I) funded by the Spanish Ministery of Science. Funding: International Programs, United States Forest Service and the Neotropical Migratory Bird Conservation Act, United States Fish and Wildlife Service and Fontagro Project. -- Research funding: Fontagro Program, Instituto Nacional de Investigaciones Agropecuarias, International Programs US Forest Service, U.S. Fish and Wildlife Service. -- |
Contenido : |
ABSTRACT.- Areas used for livestock production and dominated by native grasses represent a unique opportunity to reconcile biodiversity conservation and livestock production. However, limited knowledge of individual species' responses to rangeland management restricts our capacity to design grazing practices that favor endangered species and other priority birds. In this work, we applied Hierarchical Modelling of Species Communities (HMSC) to study individual species responses, as well as the influence of traits on such responses, to variables related to rangeland management using birds of the Rio de la Plata Grasslands as a case study. Based on presence-absence data collected in 454 paddocks across 46 ranches we inferred the response of 69 species considering imperfect detection. This degree of detail fills a major gap in rangeland management, as species-level responses can be used to achieve targeted conservation goals other than maximizing richness or abundance. We found that artificial pastures had an overall negative impact on many bird species, whereas the presence of tussocks had a positive effect, including all threatened species. Grassland specialists were in general sensitive to grass height and tended to respond positively to tussocks but negatively to tree cover. Controlling grass height via adjustments in stocking rate can be a useful tool to favor grassland specialists. To favor a wide range of bird species in ranches, a mosaic of short and tall native grasslands with patches of tussocks and trees is desirable. We also found that species-specific responses were modulated by their traits: small-sized birds responded positively to tussocks and tree cover while large species responded negatively to increasing grass height. Ground foragers preferred short grass while birds that scarcely use this stratum were not affected by grass height. Results on the influence of traits on bird responses are an important novelty in relation to previous work in rangelands and potentially increase our predicting capacity and model transferability across grassland regions. © 2023 The Ecological Society of America. MenosABSTRACT.- Areas used for livestock production and dominated by native grasses represent a unique opportunity to reconcile biodiversity conservation and livestock production. However, limited knowledge of individual species' responses to rangeland management restricts our capacity to design grazing practices that favor endangered species and other priority birds. In this work, we applied Hierarchical Modelling of Species Communities (HMSC) to study individual species responses, as well as the influence of traits on such responses, to variables related to rangeland management using birds of the Rio de la Plata Grasslands as a case study. Based on presence-absence data collected in 454 paddocks across 46 ranches we inferred the response of 69 species considering imperfect detection. This degree of detail fills a major gap in rangeland management, as species-level responses can be used to achieve targeted conservation goals other than maximizing richness or abundance. We found that artificial pastures had an overall negative impact on many bird species, whereas the presence of tussocks had a positive effect, including all threatened species. Grassland specialists were in general sensitive to grass height and tended to respond positively to tussocks but negatively to tree cover. Controlling grass height via adjustments in stocking rate can be a useful tool to favor grassland specialists. To favor a wide range of bird species in ranches, a mosaic of short and tall native grasslan... Presentar Todo |
Palabras claves : |
ÁREA DE RECURSOS NATURALES, PRODUCCIÓN Y AMBIENTE - INIA; Body size; Conservation; Foraging behavior; Imperfect detection; Multispecies occupancy models; Native grasses; Target species. |
Asunto categoría : |
P01 Conservación de la naturaleza y recursos de La tierra |
Marc : |
LEADER 04142naa a2200301 a 4500 001 1064446 005 2024-01-30 008 2023 bl uuuu u00u1 u #d 022 $a1051-0761 024 7 $a10.1002/eap.2933$2DOI 100 1 $aALDABE, J. 245 $aBird species responses to rangeland management in relation to their traits$bRio de la Plata Grasslands as a case study.$h[electronic resource] 260 $c2023 500 $aArticle history: Manuscript received: 25 April 2023; Manuscript revised: 31 August 2023; Manuscript accepted: 04 October 2023; Accepted manuscript online: 20 November 2023; Version of Record online: 20 December 2023. -- Correspondence: Aldabe, J.; Departamento de Sistemas Agrarios y Paisajes Culturales, Centro Universitario Regional del Este, Universidad de la República, Rocha, Uruguay; email:joaquin.aldabe@gmail.com -- Funding: This paper was developed in the context of J. Aldabe PhD at Ciencias Agrarias Postgraduate Program, Agronomy Faculty, Universidad de la República. TML was supported by a Juan de la Cierva Incorporación Fellowship (IJC2020-043765-I) funded by the Spanish Ministery of Science. Funding: International Programs, United States Forest Service and the Neotropical Migratory Bird Conservation Act, United States Fish and Wildlife Service and Fontagro Project. -- Research funding: Fontagro Program, Instituto Nacional de Investigaciones Agropecuarias, International Programs US Forest Service, U.S. Fish and Wildlife Service. -- 520 $aABSTRACT.- Areas used for livestock production and dominated by native grasses represent a unique opportunity to reconcile biodiversity conservation and livestock production. However, limited knowledge of individual species' responses to rangeland management restricts our capacity to design grazing practices that favor endangered species and other priority birds. In this work, we applied Hierarchical Modelling of Species Communities (HMSC) to study individual species responses, as well as the influence of traits on such responses, to variables related to rangeland management using birds of the Rio de la Plata Grasslands as a case study. Based on presence-absence data collected in 454 paddocks across 46 ranches we inferred the response of 69 species considering imperfect detection. This degree of detail fills a major gap in rangeland management, as species-level responses can be used to achieve targeted conservation goals other than maximizing richness or abundance. We found that artificial pastures had an overall negative impact on many bird species, whereas the presence of tussocks had a positive effect, including all threatened species. Grassland specialists were in general sensitive to grass height and tended to respond positively to tussocks but negatively to tree cover. Controlling grass height via adjustments in stocking rate can be a useful tool to favor grassland specialists. To favor a wide range of bird species in ranches, a mosaic of short and tall native grasslands with patches of tussocks and trees is desirable. We also found that species-specific responses were modulated by their traits: small-sized birds responded positively to tussocks and tree cover while large species responded negatively to increasing grass height. Ground foragers preferred short grass while birds that scarcely use this stratum were not affected by grass height. Results on the influence of traits on bird responses are an important novelty in relation to previous work in rangelands and potentially increase our predicting capacity and model transferability across grassland regions. © 2023 The Ecological Society of America. 653 $aÁREA DE RECURSOS NATURALES, PRODUCCIÓN Y AMBIENTE - INIA 653 $aBody size 653 $aConservation 653 $aForaging behavior 653 $aImperfect detection 653 $aMultispecies occupancy models 653 $aNative grasses 653 $aTarget species 700 1 $aMORÁN-LÓPEZ, T. 700 1 $aSOCA, P. 700 1 $aBLUMETTO, O. 700 1 $aMORALES, J.M. 773 $tEcological Applications, 2023, ee2933. https://doi.org/10.1002/eap.2933
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